Review



ipo13b sgrna2  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Addgene inc ipo13b sgrna2
    Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific <t>ipo13b</t> mutants at 5 dpf labelled using the
    Ipo13b Sgrna2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipo13b+sgrna2/pU6a%3AsgRNA%232+(Plasmid+%2364246)/pm38413580-398-16-20
    Average 92 stars, based on 3 article reviews
    ipo13b sgrna2 - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons."

    Article Title: Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons.

    Journal: Nature communications

    doi: 10.1038/s41467-024-45908-6

    Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the
    Figure Legend Snippet: Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the

    Techniques Used: Control, Comparison, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons
    Article Snippet: To clone the sgRNA sequences into the U6 expression vectors, annealed oligos containing the sgRNA and BsmBI sequences were generated using the following primer pairs: 5’-TTCGGTGCCTGAGGCCTGGCCGG & 5’-AAACCCGGCCAGGCCTCAGGCAC, 5’-TTCGGTGCTGACGGTTCTTCCAG & 5’-AAACCTGGAAGAACCGTCAGCAC, 5’-TTCGGGAGTCAAAGACATTGTGA & 5’-AAACTCACAATGTCTTTGACTCC. .. 200 μM of each oligo were mixed together in a 20 μl reaction with NEB Buffer 2.1 and incubated at 95 o C for 5 min, then decreased to 50 o C at 0.1 o C/s, followed by a 10 min incubation at 50 o C. The annealed oligos were then ligated into U6 promotor-based cassettes: ipo13b sgRNA1 into pU6a:sgRNA#1 (Addgene #64245), ipo13b sgRNA2 into pU6a:sgRNA#2 (Addgene #64246), and ipo13b sgRNA3 into pU6b:sgRNA#3 (Addgene #64247). .. Ligation reactions were carried out by mixing 1 μL 10× NEB CutSmart buffer, 1 μL T4 DNA ligase buffer, 100 ng U6 plasmid, 1 μl annealed oligos, 0.3 μL T4 DNA ligase, 0.3 μL BsmBI, 0.2 μl PstI, and 0.2 μL SalI in a 10 μL reaction, then incubating for 3 cycles of 37 °C for 20 min and 16 °C for 15 min.



    Similar Products

    ipo13b sgrna2  (Addgene inc)
    92
    Addgene inc ipo13b sgrna2
    Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific <t>ipo13b</t> mutants at 5 dpf labelled using the
    Ipo13b Sgrna2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipo13b+sgrna2/pU6a%3AsgRNA%232+(Plasmid+%2364246)/pm38413580-398-16-20
    Average 92 stars, based on 1 article reviews
    ipo13b sgrna2 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the

    Journal: Nature communications

    Article Title: Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons.

    doi: 10.1038/s41467-024-45908-6

    Figure Lengend Snippet: Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the

    Article Snippet: The annealed oligos were then ligated into U6 promotor-based cassettes: ipo13b sgRNA1 into pU6a:sgRNA#1 (Addgene #64245), ipo13b sgRNA2 into pU6a:sgRNA#2 (Addgene #64246), and ipo13b sgRNA3 into pU6b:sgRNA#3 (Addgene #64247).

    Techniques: Control, Comparison, Two Tailed Test